Extraction of Recombinant Periplasmic Proteins Under Industrially Relevant Process Conditions: Selectivity and Yield Strongly Depend on Protein Titer and Methodology

On this work, we tried to determine a technique for the selective extraction of periplasmic endogenously expressed proteins, which is relevant at an industrial scale. For this goal, we used an expression mannequin that permits co-expression of two fluorescent proteins, every of which is particularly focused to both the cytoplasm…