Identification and in vitro Characterization of a Novel Phage Endolysin that Targets Gram-Negative Bacteria

Most double-stranded (ds) DNA phages make the most of holin proteins to secrete endolysin for host peptidoglycan lysis. In distinction, a number of holin-independent endolysins with secretion sequences or signal-arrest-release (SAR) sequences are secreted through the Sec pathway. On this research, we characterised a novel lysis protein (M4Lys) encoded by the dsDNA phage…